The aim of this study was to obtain new electrochemically modified electrodes with graphene and Au, Pt, Ag particles considering graphite (GP) and glassy carbon (GC) substrate by applying the chronoamperometry technique in order to develop the detection protocol of tetracycline (TC) considered as emerging pollutant in water, using cyclic voltammetry (CV) technique. The graphite-based substrate used for Ag/ Au/ Pt electrodeposition led to the electrode compositions on which TC oxidation process was not diffusion-controlled and as consequence, TC detection failed. TC detection protocols were developed for all Ag/Au /Pt electrodeposited GC and GC-GP electrodes. Better limits of TC detection was achieved for Ag electrodeposited on GC-GP at the cathodic potential of 0.460 V/SCE.